Detection and molecular characterization of some viruses on Cucurbitaceae plants growing in Hatay province by DAS-ELISA and RT-PCR methods

Aims: In this study, it was aimed to investigate the occurrence of cucurbit yellow stunting disorder virus (CYSDV), chlorotic yellows (CCYV) and beet pseudo-yellows (BPYV) in cucumber, zucchini melon plants grown Hatay province by Double Antibody Sandwich-Enzyme Linked Immuno Sorbent Assay (DAS-ELISA) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) methods, analyse phylogenetic relationships identified isolates.Methods Results: Totally 90 symptomatic non-symptomatic samples were collected from province. DAS-ELISA RT-PCR methods used presence CCYV method for CYSDV BPYV. The most common symptoms observed are leaf shrinkage, deformation, wrinkles, curling vein clearing. According results, CCYC not found tested. As a result analysis, BPYV could tested, but PCR amplicons 364 bp amplifying heat shock protein 70h (HSP70h) gene obtained 11 (9 zucchini, 2 melons). bidirectional sequence analysis 10 izolates, determined that nucleotide sequences highly similar (99%) isolates registered GeneBank. reported first time growing province.Conclusions: detected be any fact 12.22% tested infected with highlights need using healthy plant materials.Significance Impact Study: proven analyses